Sickle Cell Screen:
Fasting Required: No
Lab: Quest Diagnostics
Specimen: Serum
Results: Average processing time 1-3 days
Note: Result turnaround times are an estimate and are not guaranteed. Our reference lab may need additional time due to weather, holidays, confirmation/repeat testing, or equipment maintenance.
Description: This is a screening test to determine the presence of sickling hemoglobins. (e.g. hemoglobin-s, hemoglobin c-Harlem). It is important to detect Hb-S in order to determine which individuals are at risk of crisis when exposed to prolonged anoxia such as may occur during surgery, athletic programs or high altitude conditions.
People with sickle cell anemia (Hgb S/S) have the hemoglobin S gene mutation on both of their beta globin genes, whereas people with sickle cell trait (Hgb A/S) have the mutation on only one beta globin gene. People with sickle cell trait are considered carriers and are usually asymptomatic. However, sickle cell formation leading to vascular occlusion can occur during high fever and under conditions of significant hypoxia. Differentiating sickle cell anemia from the trait is important for clinical management and genetic counseling of the individual.
Sickle cell disease refers to any condition that causes a sickling disorder. Sickle cell anemia is one type of sickle cell disease. It is caused by the presence of a Hgb S on both beta globin genes. Other types of sickle cell disease are caused by presence of Hgb S on one beta globin gene and Hgb C, Hgb D-Punjab (D-Los Angeles), Hgb O-Arab, Hgb C-Harlem, Hgb Lepore, or beta thalassemia on the other beta globin gene.
The sickle cell solubility test is a qualitative test based on the relative insolubility of hemoglobin S compared to other hemoglobin variants. A positive test indicates the presence of hemoglobin S or a non-S sickling hemoglobin. Results should be confirmed by high-performance liquid chromatography (HPLC) (Hemoglobinopathy Evaluation, test code 35489).
A positive test is consistent with sickle cell trait, sickle cell anemia and hemoglobin S in combination with another hemoglobin variant, e.g. hemoglobin C-Harlem. Positive results can also occur with other sickling hemoglobins like Hgb S Travis, Hgb C Ziguinchor as well as with high concentrations of Hgb Bart’s. A false positive result can also be seen in cryoglobulinemia and certain medical conditions, e.g., polycythemia and hypergammaglobulinemia (e.g. multiple myeloma), when nucleated red blood cells are present in the peripheral blood.
This test does not differentiate sickle cell trait from sickle cell anemia nor is it intended as a screen for other significant hemoglobin variants that combine with hemoglobin S, e.g. hemoglobin C and hemoglobin D.
Alternative Name(s): Hemoglobin S
Lab: Quest Diagnostics
Specimen: Serum
Results: Average processing time 1-3 days
Note: Result turnaround times are an estimate and are not guaranteed. Our reference lab may need additional time due to weather, holidays, confirmation/repeat testing, or equipment maintenance.
Description: This is a screening test to determine the presence of sickling hemoglobins. (e.g. hemoglobin-s, hemoglobin c-Harlem). It is important to detect Hb-S in order to determine which individuals are at risk of crisis when exposed to prolonged anoxia such as may occur during surgery, athletic programs or high altitude conditions.
People with sickle cell anemia (Hgb S/S) have the hemoglobin S gene mutation on both of their beta globin genes, whereas people with sickle cell trait (Hgb A/S) have the mutation on only one beta globin gene. People with sickle cell trait are considered carriers and are usually asymptomatic. However, sickle cell formation leading to vascular occlusion can occur during high fever and under conditions of significant hypoxia. Differentiating sickle cell anemia from the trait is important for clinical management and genetic counseling of the individual.
Sickle cell disease refers to any condition that causes a sickling disorder. Sickle cell anemia is one type of sickle cell disease. It is caused by the presence of a Hgb S on both beta globin genes. Other types of sickle cell disease are caused by presence of Hgb S on one beta globin gene and Hgb C, Hgb D-Punjab (D-Los Angeles), Hgb O-Arab, Hgb C-Harlem, Hgb Lepore, or beta thalassemia on the other beta globin gene.
The sickle cell solubility test is a qualitative test based on the relative insolubility of hemoglobin S compared to other hemoglobin variants. A positive test indicates the presence of hemoglobin S or a non-S sickling hemoglobin. Results should be confirmed by high-performance liquid chromatography (HPLC) (Hemoglobinopathy Evaluation, test code 35489).
A positive test is consistent with sickle cell trait, sickle cell anemia and hemoglobin S in combination with another hemoglobin variant, e.g. hemoglobin C-Harlem. Positive results can also occur with other sickling hemoglobins like Hgb S Travis, Hgb C Ziguinchor as well as with high concentrations of Hgb Bart’s. A false positive result can also be seen in cryoglobulinemia and certain medical conditions, e.g., polycythemia and hypergammaglobulinemia (e.g. multiple myeloma), when nucleated red blood cells are present in the peripheral blood.
This test does not differentiate sickle cell trait from sickle cell anemia nor is it intended as a screen for other significant hemoglobin variants that combine with hemoglobin S, e.g. hemoglobin C and hemoglobin D.
Alternative Name(s): Hemoglobin S