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Trio-Smart SIBO/IMO (Glucose)-Gemelli Biotech Lab Kit:

Note: This is a test kit that will be mailed to you. It requires an at home breath collection.


This kit cannot be shipped through a shipping broker. It must be shipped direct to customer.

Coupon Codes not valid for this testing.

Fasting Required: Yes, 8-12 hours
Lab: Gemelli BioTech
Specimen: 9 breath samples
Results: Average processing time 7 business days
Note: Result turnaround times are an estimate and are not guaranteed. Our reference lab may need additional time due to weather, holidays, confirmation/repeat testing, or equipment maintenance.

Special Instructions:
  • If you are taking antibiotics, please consult with your provider. Your provider may want you to avoid antibiotics for a period of time prior to sample collection.
  • If tolerated, promotility drugs and laxatives should be stopped at least 7 days prior to sample collection.
  • A fasting period of 8–12 hours should be observed prior to sample collection.
  • A restricted diet should be followed on the day prior to sample collection. During this time period, restrict your diet to only meat (poultry, fish, beef or pork), tofu, eggs, potatoes and white rice, limiting your seasonings to oil, salt and pepper. In addition to water, drink only coffee or tea without any additives. This diet has been specially designed to ensure the accuracy of your test results. Do not eat or drink anything other than the items listed here. No fruits or vegetables the day before the test. Do not eat or drink during sample collection.

Included: Sugar substrate drink included. You are required to drink the full dose of 75gm of glucose solution after collecting your first sample.

Description: Trio-Smart is the only breath test available that can measure all three of the primary fermented gases in your microbiome: hydrogen, methane, and hydrogen sulfide. The Trio-Smart breath test is applicable to patients who experience bloating, gas, abdominal pain, constipation and/or diarrhea. Trio-Smart measures the levels of hydrogen, methane, and hydrogen sulfide in your breath. Elevated levels of gases can indicate small intestinal bacterial overgrowth (SIBO), intestinal methanogenic overgrowth (IMO), and/or excess hydrogen sulfide.

The Trio-Smart breath test measures the three primary fermented gases in your microbiome by way of your breath before and after a sugar substrate drink is consumed. The gases measured are hydrogen, methane, and hydrogen sulfide.

Hydrogen is produced when certain bacteria consume sugars.
  • Hydrogen levels are considered abnormal when they rise greater than =20 ppm (parts per million) from the baseline within 90 minutes.
Methane is produced when archaea (single-celled organisms similar to bacteria) consume hydrogen.
  • Methane levels are considered abnormal if they reach =10 ppm (parts per million) at any point during the breath test.
Hydrogen sulfide is produced when sulfate-reducing organisms consume hydrogen.
  • Hydrogen sulfide levels are considered abnormal if they reach =3ppm (parts per million) at any point during the breath test.